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BARTLETT, DOUGLAS

Professor of Marine Microbial Genetics

Degrees:

• B.S., Valparaiso University, Indiana
• Ph.D., University of Illinois at Chicago

Research Interests:

• Deep-sea microbial diversity and ecology
• Adaptations of deep-sea microbes to high pressure and low temperature
• Microbial genetics, genomics, functional genomics

Courses Recently Taught:

• SIO 242B: Marine Biotechnology II
• SIO181: Marine Biochemistry
• SIO242: Marine Biotechnology

Honors and Awards:

• Faculty of 1000 Biology member, 2008
• Miller Lecturer, 2005
• Australia Society for Microbiology Lecturer, 2003

Mentoring:

• Marietou, Angeliki (Post Doc; 2009-2010)
• Nguyen, Alice (Masters; 2009-2010)
• Cao, Yi (Graduate; 2008-2010)
• Kerman, Ian (Graduate; 2006-2008)
• Leon-Zayas, Rosa (Graduate; 2006-)
• Saini, Simran (Graduate; 2006-2007)
• Eloe, Emiley (Graduate; 2005-)
• Ben-Haim Rozenblat , Yael (Post Doc; 2003-2003)
• Millikan, Debbie (Post Doc; 2002-2002)
• Adams, Cameron (Graduate; 2001-2003)
• Egan, Su (Post Doc; 2001-2001)
• Lauro, Federico (Graduate; 2001-2007)
• Mueller, Ryan (Graduate; 2001-2007)
• Purdy, Alexandra (Graduate; 2001-2007)
• Wardlaw, George (Graduate; 2001-2003)
• Valentine, David (Post Doc; 2000-2003)
• Allen, Eric (Graduate; 1997-2002)
• Bidle, Kelly (Post Doc; 1997-2001)
• Chilukuri, Lakshmi (Graduate; 1992-1998)
• Tobal, Gabriela (Graduate; 1991-1993)
• Welch, Tim (Graduate; 1990-1996)
• Chi, Ellen (Graduate; 1989-1995)

Nagata, T., Tamburini, C., Arístegui, J., Baltar, F., Bochdansky, A., Fonda-Umani, S., Fukuda, H., Gogou, A., Hansell, D. A. , Hansman, R. J., Herndl, G. J., Panagiotopoulos, C., Reinthaler, T., Sohrin, R., Verdugo, P., Yamada, N., Yamashita, Y., Yokokawa, T., Bartlett, D. H. 2010. Emerging concepts on microbial processes in the bathypelagic ocean–ecology, biogeochemistry, and genomics. Deep-Sea Research II: in press (doi:10.1016/j.dsr2.2010.02.019).

Bartlett, D. H. and Kerman, I. 2010. Contributions of large-scale DNA sequencing efforts to the understanding of low temperature piezophiles. In extremophiles handbook (Horikoshi, K., Antranikian, G., Bull, A., Robb, F., and Stetter, K., eds.), Springer-Verlag, Heidelberg, Germany), in press.

Ziad W. E.-H., Allcock, D., Tryfona, T., Lauro, F. M., Sawyer, L., Bartlett, D. H., Ferguson, G. P. 2010. Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9. In high-pressure bioscience and biotechnology (Bartlett, D. H., ed.), Blackwell Publishing, Boston, MA, pp. 143-148.

Bartlett, D. H. 2010. Introduction to high-pressure bioscience and biotechnology. In high-pressure bioscience and biotechnology (Bartlett, D. H., ed.), Blackwell Publishing, Boston, MA, pp. 1-5.

Bartlett, D. H. 2010. High-pressure bioscience and biotechnology. Annals of the New York Academy of Sciences. Blackwell Publishing, Boston, MA

Purdy, A. E., Balch D. Lizárraga-Partida, M. L., Islam, M. S., Martinez-Urtaza, J., Huq, A., Colwell. R. R., and Bartlett, D. H 2010. Diversity and distribution of cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae. Environmental Microbiology Reports 2:198-207.

Yoshioka, H., Maruyama, A., Nakamura, T., Higashi, Y., Fuse, H., Sakata, S., Bartlett, D. H. 2010. Activities and distribution of methanogenic and methane-oxidizing microbes in marine sediments from the Cascadia Margin. Geobiology 8:223-233.

Bartlett, D. H. 2009. Microbial life in the trenches. Marine Technology Society Journal 43: 128-131.

Lowery, C., Park, J., Gloeckner, C., Meijler, M., Mueller, R., Boshoff, H., Ulrich, R., Barry, C., Bartlett , D., Kravchenko, V. 2009. Defining the mode of action of tetramic acid antibacterials derived from Pseudomonas aeruginosa quorum sensing signals. Journal of the American Chemical Society 131: 14473-14479.

El-Haj, Z. W., Tryfonay, T., Allcock, D. J., Hasan, F., Lauro, F. M., Sawyer, L., Bartlett, D. H. and Ferguson, G. P. 2009. Importance of proteins controlling the initiation of DNA replication in the growth of the high pressure-loving bacterium, Photobacterium profundum SS9. J. Bacteriol. 191: 6383-6393.

Prieur, D., Jebbar, M., Bartlett, D., Kato, C., and Oger, P. 2009. Piezophilic prokaryotes. In Comparative high pressure biology (Sebert, P., ed.) Science Publishers, Enfield, New Hampshire, p. 281-318.

Linke, K., Periasamy, N., Eloe, E. A., Ehrmann, M., Winter, R., Bartlett, D. H. and Vogel, R. F. 2009. Influence of membrane organization on the dimerization ability of ToxR from Photobacterium profundum under high hydrostatic pressure. High Pressure Research 29:431-442.

Mueller, R. S., Beyhan, S., Saini,S. G., Yildiz, F. H., Bartlett, D. H. 2009. Indole acts as an extracellular cue regulating gene expression in Vibrio cholerae. J. Bacteriol. 191: 3504-3516.

Eloe, E. E. , Lauro, F. M., Vogel, R. F, Bartlett, D. H. 2008. The deep-sea bacterium Photobacterium profundum SS9 utilizes separate motility systems for swimming and swarming at high pressure. Appl. Environ. Microbiol. 74:6298-6305.

Xiao, X., Wang, P., Zeng, X., Chen, M., Bartlett, D. H. and Wang, F. 2008. Environmental Adaptation: Genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3. PLoS ONE 3(4):e1937.

Bartlett, D. H., Ferguson, G. and Valle, G. 2008. Adaptations of the Psychrotolerant Piezophile Photobacterium profundum Strain SS9. In high pressure microbiology (Michiels, C, Bartlett, D. H., and Aertsen, A, eds.), American Society for Microbiology Press, Washington, D. C., pp. 319-337.

Bartlett, D. H. 2008. Introduction to deep-sea microbiology. In high pressure microbiology (Michiels, C, Bartlett, D. H., and Aertsen, A, eds.), American Society for Microbiology Press, Washington, D. C., pp. 195-201.

Michiels, C, Bartlett, D. H., Aertsen, A (eds.). 2008. High pressure microbiology. American Society for Microbiology Press, Washington, D. C.

Jørgensen, R., Purdy, A. E. , Fieldhouse, R. J., Kimber, M. S. , Bartlett, D. H. and Merrill, A. R. 2008. Cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae. J. Biol. Chem. 283: 10671-10678. (Bartlett and Merrill are co-contributing authors).

Lauro, F. M. , Tran, K. , Vezzi, A., Vitulo, N., Valle, G. , Bartlett, D. H. 2008. Large-scale transposon mutagenesis of Photobacterium profundum SS9 reveals new genetic loci important for growth at low temperature and high pressure. J. Bacteriol. 190:1699-1709.

Mueller R. S, McDougald D., Cusumano D., Sodhi N., Kjelleberg S., Azam F., Bartlett D. H. 2007. Vibrio cholerae strains possess multiple strategies for abiotic and biotic surface colonization. J. Bacteriol. 189:5348-60 (cover story).

Makri, S.. Purdy, A. E., Bartlett, D. H., Fierer, J. 2007. Pathogenicity of environmental isolates of V. cholerae in mice. Microbes and Infection 9: 1351-1358.

Xiao, X., Wang, P., Zeng, X., Bartlett, D. H., Wang, F. 2007. Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment. Int. J. Syst. Evol. Microbiol. 57:60-65.

Bartlett, D. H., Lauro, F. M. and Eloe, E. A. 2007. Microbial adaptation to high pressure In physiology and biochemistry of extremophiles (Gerday, C. and Glandsdorf, N., eds.). American Society for Microbiology Press, Washington, D. C., pp. 333-348.

Lauro, F. M. Chastain, R. A., Blankenship, L. E. Yayanos, A. A. and Bartlett, D. H. 2007. The unique 16S rRNA genes of piezophiles reflect both phylogeny and adaptation. Appl. Environ. Microbiol. 73:838-845.

Lauro, F. M. and Bartlett, D. H. 2007. Prokaryotic lifestyles in deep-sea habitats. Extremophiles 12:15-25. Simonato F, Campanaro S, Lauro FM, Vezzi A, D'Angelo M, Vitulo N, Valle G, Bartlett DH. 2006. Piezophilic adaptation: a genomic point of view. J Biotechnol. 126:11-25.

Worden A. Z., Cuvelier M. L., Bartlett D. H. 2006. In-depth analyses of marine microbial community genomics. Trends Microbiol. 14:331-336.

Stelzer, S, Egan, S., Larsen, M. R., Bartlett, D. H. and Kjelleberg, S. 2006. Unravelling the role of the ToxR-like transcriptional regulator WmpR in the marine antifouling bacterium Pseudoalteromonas tunicata. Microbiology 152:1385-1394.

Fang, J., Uhle, M., Billmark, K., Bartlett, D. H., and Kato, C. 2006. Fractionation of carbon isotopes in biosynthesis of fatty acids by a piezophilic bacterium Moritella japonica strain DSK1. Geochimica Cosmochimica Acta. 70:1753-1760.

Bartlett, D. H. 2006. Extremophilic Vibrionaceae. In the biology of vibrios (Thompson, F. L., Austin, B. and Swings, J.; eds.) American Society for Microbiology Press, Washington D. C., pp. 156-171.

Worden, A. Z., Seidel, M., Smriga, S., Wick, A., Malfatti, F., Bartlett, D. and Azam, F. 2006. Trophic regulation of Vibrio cholerae in coastal marine waters. Environ. Microbiol. 8:21-29. Bartlett, D. H. and Azam, F. 2005. Chitin, cholera and competence. Science 310:1775-1777.

Casas, V., Miyake, J., Balsley, H., Roarke, J., Telles, S., Leeds, S., Zurita, I., Breitbart, M., Bartlett, D. H., Azam. F. and Rohwer, F. 2006. Widespread occurrence of phage-encoded exotoxin genes in terrestrial and aquatic environments. FEMS Microbiol Lett. 261: 141-149.

Valentine, D. L., Kastner, M., Wardlaw, G. D., Wang, X., Purdy, A. and Bartlett, D. H. 2005. Biogeochemical investigations of marine methane seeps, Hydrate Ridge, Oregon. J. Geophys. Res. 110: G02005, doi:10.1029/2005JG000025. Long, R., Rowley, D., Zamora, E. Bartlett, D. H. and Azam, F. 2005. Antagonistic interactions among marine bacteria impede the spread of Vibrio cholerae. Appl. Environ. Microbiol. 71: 8531-8536.

Campanaro, S. , Vezzi, A., Vitulo, N., Lauro, F. M., D’Angeo, M., Simonato, F., Cestaro, A., Malacrida, G., Bertoloni, G., Valle, G. and Bartlett, D. H. 2005. Laterally transferred elements and high pressure adaptation in Photobacterium profundum strains. BMC genomics 6:122. (15 pages).

Lanoil, B. D., La Duc, M. T., Wright, M., Kastner, M., Nealson, K. H., Bartlett, D. 2005. Archaeal diversity in ODP legacy borehole 892b and associated seawater and sediments of the Cascadia Margin. FEMS Microbiol. Ecol. 54:167-177.

Lauro, F. M., Eloe, E. A., Liverani, N., Bertoloni, G., and D. H. Bartlett. 2005. Conjugal vectors for cloning, expression, and insertional mutagenesis in Gram-negative bacteria. BioTechniques 38:708-710.

Purdy. A., Rohwer, F., Edwards, R., Azam, F., and Bartlett, D. H. 2005. A glimpse into the expanded genome content of the Vibrio cholerae species through the identification of genes present in environmental strains. J. Bacteriol. 187:2992-3001. Vezzi, A., Campanaro, S., D’Angelo, M., Simonato, F., Vitulo, N., Lauro, F. M., Cestaro, A., Malacrida, G., Simionati, B., Cannata, N., Romualdi, C., Bartlett D. H., and Valle, G. 2005. Life at depth: Photobacterium profundum genome sequence and expression analysis. Science 307:1459-1461.

Allen, E. E. and Bartlett, D. H. 2005. Piezophiles: microbial adaptation to the deep-sea environment, in Extremophilies (Life under extreme enviromental condition, from Encyclopedia of Life Support Systems (EOLSS), developed under the auspices of the UNESCO, Eolss Publishers, Oxford ,UK, [Link to publication (22 pages).

Lauro, F. M., Bertoloni, G., Obraztsova, A., Kato, C., Tebo, B. M. and Bartlett, D. H. Pressure effects on Clostridium strains isolated from a cold deep-sea environment. Extremophiles 8:169-173. 2004.

Martin, D. D., Bartlett, D. H. and M. F. Roberts. Solute accumulation in the deep-sea bacterium Photobacterium profundum. Extremophiles 6:507 2002 (9 pages)

Kato, C, Li, L., Nakasone, K. and Bartlett, D. H. 2002. Marine microbiology: deep sea adaptations. In Y. Taniguchi, Stanley, H. E., Ludwif, H. (ed.) Biological systems under extreme conditions: structure and function. Tokyo, Springer. p. 205 (16 pages)

Allen, E. E. and Bartlett, D. H. Structure and regulation of the omega-3 polyunaturated fatty acid synthase genes from the deep-sea bacterium Photobacterium profundum strain SS9. Microbiology 148:1903 2002 (10 pages) [Soc. for General Microbiol.]

Carson, B., Kastner, M., Bartlett, D., Jaeger, J., Jannasch, H., Weinstein, Y. Implications of active carbon flux from the Cascadia accretionary prism: results from long-term, in-situ measurements at ODP site 892b. Mar. Geol. 198:159 2003 (21 pages)

Chilukuri, L. N., Bartlett, D. H. And Fortes, P. A. G. Comparison of high pressure-induced dissociation of single-stranded DNA-binding protein (SSB) from high pressure-sensitive and high pressure-adapted marine Shewanella species. Extremophiles 6:377 2002 (7 pages)

Bartlett, D. H. pressure effects on in vivo microbial processes. Biochem. Biophys Acta 1595: 367 2002 (14 pages) [Elsevier Science B.V.]

Bidle, K. A. and D. H. Bartlett. RNA Arbitrarily primed PCR survey of genes regulated by ToxR and ToxS in the deep-sea bacterium Photobacterium profundum strain SS9. J. Bacteriol. 183: 1688 2001. (5 pages)

Allen, E. E. and D. H. Bartlett. FabF is required for Piezoregulation of cis-vaccenic acid levels and piezophilic growth of the deep-sea bacterium Photobacterium profundum strain SS9. J. Bacteriol. 182: 1264 2000. (8 pages)

Bartlett, D. H. and Roberts, M. F. Osmotic stress. In Encyclopedia of microbiology (J. Lederberg, ed.), volume 3, second edition, Academic Press, San Diego. pp. 501-516. 2000.

Kastner, M. A., B. Carson, D. H. Bartlett, J. Jaeger, K. Bidle and H. Jannasch. 2000. An in situ experiment of methane sequestration as gas hydrate, authigenic carbonate, and lossto the water column and/or atmosphere. In gas hydrates challenges for the future (G.D. Holder and P. R. Bishnoi, eds.). The New York Academy of Sciences, New York, pp. 32-38.

Bidle, K. A., M. Kastner and D. H. Bartlett. A phylogenetic analysis of microbial communities associated with methane hydrate containing marine fluids and sediments in the Cascadia margin (ODP site 892b). FEMS Microbiol. Lett. 177: 101 1999. (8 pages)

Bartlett, D. H. 1999. Microbial adaptations to the psychrosphere/piezosphere, p. 185-202. In D. H. Bartlett (ed.) Molecular marine microbiology. Horizon Scientific Press, Norfolk. Also published in J. Mol. Microbiol. Biotechnol. 1:93 1999 (8 pages) Bidle, K. A. and D. H. Bartlett. RecD function is required for high-pressure growth of a deep-sea bacterium. J. Bacteriol. 181: 2330 1999. (8 pages)

Allen, E. E., D. Facciotti and D. H. Bartlett. Monounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium Photobacterium profundum SS9 at high pressure and low temperature. Appl. Environ. Microbiol. 65: 1710 1999. (11 pages).

Bartlett, D. H. and K. A. Bidle. Membrane-based adaptations of deep-sea piezophiles. In (J. Seckbach, ed.) Enigmatic microbes and life in extreme environments. Kluwer Academic Publishers, The Netherlands, pp. 501-512. 1999.

Welch, T. J. and D. H. Bartlett. Identification of a regulatory protein required for pressure-responsive gene expression in the deep-sea bacterium Photobacterium profundum species strain SS9. Mol. Microbiol. 27:977 1998. (9 pages)

Kato, C. and D. H. Bartlett. The molecular biology of barophilic bacteria. Extremophiles 1:111 1997. (6 pages)

Chilukuri, L.N. and D.H. Bartlett. Isolation and characterization of the gene encoding single-stranded DNA-binding protein (SSB) from four marine Shewanellas differing with regard to temperature and pressure adaptation. Microbiology. 143: 1163 1997. (11 pages)

Hauben, K., D. Bartlett, C. Soontjens, K. Cornelis, E. Wuytack and C. Michiels. Escherichia coli mutants resistant to inactivation by high hydrostatic pressure. Applied and Environ. Microbiology 63: 945. 1997. (6 pages)

Chilukuri, L. N. and D. H. Bartlett. High pressure modulation of Escherichia coli DNA gyrase activity. Biochem. Biophys. Res. Comm. 239: 552 1997. (5 pages) Bartlett, D.H. and T.J. Welch. Role of cAMP receptor protein and ToxR/S proteins in pressure sensing and pressure adaptation in the deep-sea bacterium Photobacterium species strain SS9. In: High Pressure Research in the Biosciences and Biotechnology (K. Heremans, ed.) Leuven University Press, Leuven, Belgium. 1997.

Welch, T.J. and D.H. Bartlett. Cloning, sequencing and overexpression of the gene encoding malate dehydrogenase from the deep-sea bacterium Photobacterium species strain SS9. Biochimica et Biophysica Acta. 1350: 41 1996. (6 pages)

Bartlett, D.H., E. Chi and T.J. Welch. High pressure sensing and adaptation in the deep-sea bacterium Photobacterium species strain SS9. In: High Pressure Bioscience and Biotechnology (R. Hayashi and C. Balny, eds.) Elsevier Science, Tokyo. 1996

Welch, T.J. and D.H. Bartlett. Isolation and characterization of the structural gene for OmpL, a pressure-regulated porin-like protein from the deep-sea bacterium Photobacterium species strain SS9. J. Bacteriol. 178: 5027. 1996 (5 pages)

Bartlett, D.H., C. Kato and K. Horikoshi. High pressure influences on gene and protein expression. Res. Microbiol. 146: 697. 1995 (10 pages)

Chi, E. and D.H. Bartlett. An rpoE-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep-sea bacterium Photobacterium sp. strain SS9. Molecular Microbiology 17: 713. 1995 (14 pages)

Bartlett, D.H. and T.J. Welch. ompH gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium Photobacterium species strain SS9. J. Bacteriology 177: 1008. 1995 (9 pages)

Bartlett, D.H. and Chi, E. Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium species strain SS9. Archives Microbiol. 162: 323. 1994 Bartlett, D.H. Microbial life at high pressures. Science Progress 76: 479. 1994 (17 pages)

Welch, T.J., Farewell, A., Neidhardt, F. C. and Bartlett, D.H. Stress response of Escherichia coli to elevated hydrostatic pressure. J. Bacteriol. 175: 7170. 1993 (8 pages) Chi, E. and Bartlett, D.H. Use of a reporter gene to follow high pressure signal transduction in the deep-sea bacterium Photobacterium SS9. J. Bacteriol. 175: 7533. 1993 (8 pages)

Bartlett, D.H., Wright, M.E. and Chi, E. Sequence of the ompH gene from the deep-sea bacterium Photobacterium SS9. Gene: 131: 125. 1993 (4 pages)

Bartlett, D.H. Pressure sensing in deep sea bacteria. Res. Microbiol. 142: 923. 1991 Bartlett, D.H., Wright, M.E., Yayanos, A.A., and Silverman, M. Isolation of a gene regulated by hydrostatic pressure in a deep-sea bacterium. Nature 342: 572. 1989 (3 pages)

Bartlett, D.H. and Silverman, M. Nucleotide sequence of IS492: a novel insertion sequence causing variation in extracellular polysaccharide production in the marine bacterium Pseudomonas atlantica. J. Bacteriol. 171: 1763. 1989 (4 pages)

Simon, M., Belas, R., McCarter, L., Bartlett, D. and Silverman, M. Microbial role in biodeterioration. In: Marine Biodeterioration: Advanced Techniques Applicable to the Indian Ocean. Edited by the American Institute of Biological Sciences. Oxford and IBH Publishing, New Delhi, India, p. 265. 1988 (22 pages)

Belas, R., Bartlett, D., and Silverman, M. Cloning and gene replacement mutagenesis of a Pseudomonas atlantica agarase gene. Appl. Environ. Microbiol. 54: 30. 1988 (8 pages)

Bartlett, D.H., Wright, M.E., and Silverman, M. Variable expression of extracellular polysaccharide in the marine bacterium Pseudomonas atlantica is controlled by genome rearrangement. Proc. Natl. Acad. Sci. U.S.A. 85: 3923. 1988 (5 pages)

Bartlett, D.H., Frantz, B.E., and Matsumura, P. Flagellar transcriptional activators F1bB and F1aI: Gene sequences and 5'consensus sequences of operons under F1bB and F1aIcontrol. J. Bacteriol. 170: 1575. 1988 (7 pages)

Bartlett, D.H. and Matsumura, P. Behavioral responses to chemical cues by bacteria. J. Chemical Ecology 12 (5): 1230. 1986 (13 pages)

Bartlett, D.H. and Matsumura, P. Identification of Escherichia coli region III flagellar gene products and description of two new flagellar genes. J. Bacteriol. 160: 577. 1984 (8 pages)