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TSUJI, FREDERICK

Research Biochemist

Degrees:

• B.A., Cornell University
• M.S., Cornell University
• Ph.D., Cornell University

Research Interests:

• Bioluminescence
• Aequorin
• Green Fluorescent Protein (GFP)
• Energy Transfer
• Calcium Ion

Honors and Awards:

• Who's Who in Science & Engineering, 10th Anniversary Edition, 2008
• Who's Who in the World, 25th Anniversary Edition, Marquis, 2008
• Who's Who in America, 61st Edition, Marquis, 2007

Inouye, S., Ogawa, H., Yasuda, K., Umesono, K. and Tsuji, F. I. A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator. Gene 189 (1997) 159- 162.

Kojima, S., Hirano, T., Niwa, H., Ohashi, M., Inouye, S. and Tsuji, F. I. Mechanism of the redox reaction of the Aequorea green fluorescent protein (GFP). Tetrahedron Lett. 38 (1997) 2875- 2878.

Nishiuchi, Y., Inui, T., Nishio, H,, Bódi, J., Kimura, T., Tsuji, F. I. and Sakakibara, S. Chemical synthesis of the precursor molecule of the Aequorea green fluorescent protein, subsequent folding, and development of fluorescence. Proc. Natl. Acad. Sci. USA 95 (1998) 13549-13554.

Yoshida, R., Sanchez-Bueno, A. Tsuji, F. I. Tracing the in vivo distribution and dynamics of cancer cells in mice by luminescence of aequorin-expressing transformants. Biochem. Biophys. Res. Commun. 242 (1998) 659- 662.

Kojima, S., Ohkawa, H., Hiranot, T., Maki, S., Niwa, H., Ohashi, M., Inouye, S. and Tsuji, F. I. Fluorescent properties of model chromophores of tyrosine substituted mutants of Aequorea green fluorescent protein (GFP). Tetrahedron Lett. 39 (1998) 5239- 5242.

Mori, I. C., Iida, H., Tsuji, F. I., Isobe, M., Uozumi, N. and Muto, S. Salicyclic acid induces a cytosolic Ca2+ elevation in yeast. Biosci. Biotechnol. Biochem. 62 (1998) 986- 989.

Inouye, S., Umesono, K. and Tsuji, F. I. Special properties of green fluorescent protein-S65A. In: Green Fluorescent Protein. Methods in Enzymology, Vol. 302, Cohn, P. M., Ed.,, pp. 444- 449, 1999. Academic Press, San Diego, CA.

Nakajima-Shimada, J., Sakaguchi, S., Tsuji, F. I., Anraku, Y. and Iida, H. Ca2+ signal is generated only once in the mating pheromone response pathway in Saccharomyces cerevisiae. Cell Struct. Function 25 (2000) 125- 131.

Kojima, S., Maki, S., Hirano, T., Ohashi, M., Tsuji, F. I. and Niwa, H. Improved syntheses of Watasenia preluciferin (coelenterazine) and Watasenia luciferin (coelenterazine disulfate), and site specific syntheses of the coelenterazine monosulfates. ITE Lett. 2 (2001) 393- 397.

Pinontoan, R., Krystofova, S., Kawano, T., Mori, I. C., Tsuji, F. I., Iida, H. and Muto, S. Phenylethylamine induces an increase in cytosolic Ca2+ in yeast. Biosci. Biotechnol. Biochem. 66 (2002) 1069- 1074.

Tsuji, F. I. Bioluminescence reaction catalyzed by membrane-bound luciferase in the “firefly squid,” Watasenia scintillans. Biochim. et Biophys. Acta, 1564 (2002) 189-197.

Tsuji, F. I. Oxidation of a cyclic tripeptide by molecular oxygen and the development of fluorescence in the Aequorea green fluorescent protein. Intl. Cong. Ser. 1233 (2002) 37- 44.

Masuda, H., Takenaka, Y., Shikamoto, Y., Kagawa, M., Mizuno, H. and Tsuji, F. I. Chromatography of isoforms of recombinant apoaequorin and method for the preparation of aequorin. Protein Express. Purif. 31, 181-187 (2003).

Tsuji, F. I. Bioluminescence reaction catalyzed by membrane-bound luciferase in the “firefly squid,” Watasenia scintillans.” Biochim. et Biophys. Acta 1564, 189-197 (2002).

Tsuji, F. I. Role of molecular oxygen in the bioluminescence of the firefly squid, Watasenia scintillans. Biochem. Biophys. Res. Commun. 338 (2005) 250- 253.