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HOLLAND, LINDA

Research Biologist

Degrees:

• B.A., Stanford University
• M.A., Stanford University
• Ph.D., University of California, San Diego

Research Interests:

• Chordate evolutionary relationships
• Developmental genetics of amphioxus
• Evolution and development

Mentoring:

• Koop, Demian (Post Doc; 2006-2008)
• Onai, Takayuki (Post Doc; 2006-2008)
• Beaster-Jones, Laura (Post Doc; 2003-2006)
• Kaltenbach, Stacy (Graduate; 2003-2008)
• Yu, Jr-Kai (Graduate; 2000-2005)
• Schubert, Michael (Graduate; 1997-2001)

2000-2008 and selected earlier references.

1995. Holland, N.D., L.Z. Holland, and Z. Kozmik, An amphioxus Pax gene, AmphiPax-1, expressed in embryonic endoderm, but not in mesoderm: implications for evolution of classI paired box genes. Mol. Mar. Biol. Biotechnol., 1995. 35: 206-214.

1997. Sequence and embryonic expression of the amphioxus engrailed gene (AmphiEn): The metameric pattern of transcription resembles that of its segment-polarity homolog in Drosophila. Development: 124:1723-1732.

1998. Isolation and developmental expression of the amphioxus Pax-6 gene (AmphiPax-6): insights into eye and photoreceptor evolution. Development 125(14):2701-10.

1998. The structure of the salp ganglion. Insights into evolution of chordate nervous systems. Philosophical Transactions of the Royal Society of London: Biological Sciences: 353:1843-1857.

1999. AmphiPax3/7, an amphioxus paired box gene: insights into chordate myogenesis, neurogenesis, and the possible evolutionary precursor of definitive vertebrate neural crest. Evol Dev. 1(3):153-65.

1999. Characterization of an amphioxus paired box gene, AmphiPax2/5/8: Developmental expression patterns in optic support cells, nephridium, thyroid-like structures and pharyngeal gill slits, but not in the midbrain-hindbrain boundary region. Development

1999. Cordate origins of the vertebrate central nervous system. Current Opinion in Neurobiology: 9(5):596-602.

1999. Holland, L.Z., M. Schubert, Z. Kozmik, and N.D. Holland, AmphiPax3/7, an amphioxus paired box gene: insights into chordate myogenesis, neurogenesis, and the possible evolutionary precursor of definitive vertebrate neural crest. Evol. & Dev., 1999. 1: 153-165.

1999. Genomic organization and evolution of actin genes in the amphioxus Branchiostoma belcheri and Branchiostoma floridae. Gene 227(1):1-10.

1999. Sequence and developmental expression of amphioxus AmphiNk2-1: insights into the evolutionary origin of the vertebrate thyroid gland and forebrain. Dev Genes Evol. 209(4):254-9.

1999. Kozmik, Z., N.D. Holland, A. Kalousova, J. Paces, M. Schubert, and L.Z. Holland, Characterization of an amphioxus paired box gene, AmphiPax2/5/8: developmental expression paterns in optic support cells, nephidium, thyroid-like structures and pharyngeal gill slits, but not in the midbrain-hindbrain boundary region. Development, 1999. 126: 1295-1304.

2000. Schubert, M., L.Z. Holland, G.D. Panopoulou, H. Lehrach, and N.D. Holland, Characterization of amphioxus AmphiWnt8: insights into the evolution of patterning of the embryonic dorsoventral axis. Evol. & Dev., 2000. 2: 85-92.

2000. Schubert, M., L.Z. Holland, and N.D. Holland, Characterization of an amphioxus Wnt gene AmphiWnt11, with possible roles in myogenesis and tail outgrowth. Genesis, 2000. 27: 1-5.

2000. Body-plan evolution in the Bilateria: early antero-posterior patterning and the deuterostome-protostome dichotomy. Curr Opin Genet Dev. 10(4):434-442.

2000. Holland, L.Z. and N.D. Holland, Developmental expression of AmphiWnt1, an amphioxus gene in the Wnt1/wingless subfamily. Dev. Genes Evol., 2000. 210: 522-524.

2000. Characterization of amphioxus AmphiWnt8: Insights into the evolution of patterning of the embryonic dorsoventral axis. Evolutionary Development: 2:85-92.

2000. Schubert, M., L.Z. Holland, and N.D. Holland, Characterization of two amphioxus Wnt genes (AmphiWnt4 and AmphiWnt7b) with early expression in the developing central nervous system. Dev. Dynam., 2000. 217: 205-215.

2000. Holland, L.Z., M. Schubert, N.D. Holland, and T. Neuman, Evolutionary conservation of the presumptive neural plate markers AmphiSox1/2/3 and AmphiNeurogenin in the invertebrate chordate amphioxus. Dev. Biol., 2000. 226: 18-33.

2000. Schubert, M., L.Z. Holland, N.D. Holland, and D.K. Jacobs, A phylogenetic tree of the Wnt genes based on all available full-length sequences, inclusing five from the cephalochordate amphioxus. Mol. Biol. Evol., 2000. 17: 1896-1903.

2000. Langlois, M.C., J.M. Vanacker, N.D. Holland, H. Escriva, C. Queva, V. Laudet, and L.Z. Holland, Amphicoup-TF, a nuclear orphan receptor of the lancelet Branchiostoma floridae, is implicated in retinoic acid signalling pathways. Dev. Genes Evol., 2000. 210: 471-482.

2001. Schubert, M., L.Z. Holland, M.D. Stokes, and N.D. Holland, Three amphioxus Wnt genes (AmphiWnt3, AmphiWnt5, and AmphiWnt6) associated with the tail bud: the evolution of somitogenesis in chordates. Dev. Biol., 2001. 240: 262-273.

2001. Holland, L.Z., Amphioxus and the evolutionary conservation of anterior-posterior patterning. Zool. Sci., 2001. 18: 458-459.

2001. Holland, L.Z. and N.D. Holland, Evolution of neural crest and placodes: amphioxus as a model for the ancestral chordate. J. Anat., 2001. 199: 85-98.

2001. Holland, L.Z. and s. more, Characterization and developmental expression of the amphioxus homolog of Notch (AmphiNotch): evolutionary conservation of multiple expression domains in amphioxus and vertebrates. Dev. Biol., 2001. 232: 493-507.

2001. Kozmik, Z., L.Z. Holland, M. Schubert, T.C. Lacalli, J. Kreslova, C. Vlcek, and N.D. Holland, Characterization of amphioxus AmphiVent, an evolutionarily conserved marker for chordate ventral mesoderm. genesis, 2001. 29: 172-179.

2002. Holland, L.Z., Heads or tails? Amphioxus and the evolution of anterior-posterior patterning in deuterostomes. Dev. Biol., 2002. 241: 209-228.

2002. Escriva, H., N.D. Holland, H. Groenmeyer, V. Laudet, and L.Z. Holland, The retinoic acid signaling pathway regulates anterior/posterior patterning in the nerve cord and pharynx of amphioxus, a chordate lacking neural crest. Development, 2002. 129: 2905-2916.

2002. Yu, J.K., L.Z. Holland, and N.D. Holland, An amphioxus nodal gene (AmphiNodal) with early symmetrical expression in the organizer and mesoderm and later asymmetrical expression associated with left-right axis formation. Evol. Dev., 2002. 4: 418-425.

2002. Yu, J.K., L.Z. Holland, M. Jamrich, I.L. Blitz, and N.D. Holland, AmphiFoxE4, an amphioxus winged helix/forkhead gene encoding a protein closely related to vertebrate thyroid transcription factor-2: expression during pharyngeal development. Evol. & Dev., 2002. 4: 9-15.

2002. Holland, L. Z. Heads or tails? Amphioxus and the evolution of anterior-posterior patterning in deuterostomes. Developmental Biology: 241(2):209-228.

2002. Kreslova, J., L.Z. Holland, M. Schubert, C. Burgtorf, V. Benes, and Z. Kozmik, Functional equivalency of amphioxus and vertebrate Pax258 transcription factors suggests that the activation of mid-hindbrain specific genes in vertebrates occurs via the recruitment of Pax regulatory elements. Gene, 2002. 282: 143-150.

2002. Yu, J.K., N.D. Holland, and L.Z. Holland, An amphioxus winged helix/forkhead gene, AmphiFoxD: insights into vertebrate neural crest evolution. Dev. Dynam., 2002. 225: 289-297.

2003. Yu, J.K., N.D. Holland, and L.Z. Holland, AmphiFoxQ2, a novel winged helix/forkhead gene, exclusively marks the anterior end of the amphioxus embryo. Dev. Genes Evol., 2003. 213: 102-105.

2003. Mazet, F., J.K. Yu, D.A. Liberles, L.Z. Holland, and S.M. Shimeld, Phylogenetic relationships of the fox (forkhead) gene family in the Bilateria. Gene, 2003. 316: 79-89.

2003. Gibson-Brown, J.J., K. Osoegawa, J.D. Mcpherson, R.H. Waterston, P.J. De Jong, D.D. Rokhsar, and L.Z. Holland, A proposal to sequence the amphioxus genome submitted to the Joint Genome Institute of the US Department of Energy. J Exp Zoolog Part B Mol Dev Evol. 300(1):5-22.

2003. Holland, L.Z. and J.J. Gibson-Brown, The Ciona intestinalis genome: when the constraints are off. BioEssays, 2003. 25: 529-532.

2003. Schubert, M., D. Meulemans, M. Bronner-Fraser, L.Z. Holland, and N.D. Holland, Differential mesodermal expression of two amphioxus MyoD family members (AmphiMRF1 and AmphiMRF2). Gene Exp. Pat., 2003. 3: 199-202.

2003. Candiani, S., J. Kreslova, V. Benes, D. Oliveri, P. Castagnola, M. Pestarino, and Z. Kozmik, Cloning and developmental expression of amphioxus Dachshund. Gene Exp. Pat., 2003. 3: 65-69.

2003. Holland, N.D., T.V. Venkatesh, L.Z. Holland, D.K. Jacobs, and R. Bodmer, AmphiNk2-tin, an amphioxus homeobox gene expressed in myocardial progenitors: insights into evolution of the vertebrate heart. Dev. Biol., 2003. 255: 128-137.

2004. Yu, J.K., N.D. Holland, and L.Z. Holland, Tissue-specific expression of FoxD reporter constructs in amphioxus embryos. Dev. Bio.. 2004. 274: 452-461.

2004. Holland, L.Z. and J.K. Yu, Cephalochordate (Amphioxus) embryos: procurement, culture, basic methods. Meth. Cell Biol., 2004. 74: 195-215.

2004. Holland, L.Z., V. Laudet, and M. Schubert, The chordate amphioxus: an emerging model organism for developmental biology. Cell. Mol. Life Sci., 2004. 61: 2290-2308.

2004. Schubert, M., N.D. Holland, H. Escriva, L.Z. Holland, and V. Laudet, Retinoic acid influences anteroposterior positioning of epidermal sensory neurons and their gene expression in a developing chordate (amphioxus). PNAS, 2004. 101(28): 10320-10325.

2005. Holland, L.Z., Non-neural ectoderm is really neural: evolution of developmental patterning mechanisms in non-neural ectoderm of chordates and the problem of sensory cell homologies. J. Exp. Zool., 2005. 304B: 304-323.

2005. Bardet, P.-L., M. Schubert, B. Horard, L.Z. Holland, V. Laudet, N.D. Holland, and J.-M. Vanacker, Expression of estrogen-receptor related receptors in amphioxus and zebrafish: Implications for the evolution of posterior brain segmentation at the invertebrate-to-vertebrate transition. Evol. Dev., 2005. 7: 223-233.

2005. Holland, L.Z., K.A. Panfilio, R. Chastain, M. Schubert, and N.D. Holland, Nuclear b-catenin promotes non-neural ectoderm and posterior cell fates in amphioxus embryos. Dev. Dynam., 2005. 233: 1430-1443.

2005. Schubert, M., J.K. Yu, N.D. Holland, H. Escriva, V. Laudet, and L.Z. Holland, Retinoic acid signaling acts via Hox1 to establish the posterior limit of the pharynx in the chordate amphioxus. Development, 2005. 132: 61-73.

2006. Marletaz, F., L.Z. Holland, V. Laudet, and M. Schubert, Retinoic acid signaling and the evolution of chordates. Int. J. Biol. Sci., 2006. 2: 38-47.

2006. Schubert, M., N.D. Holland, V. Laudet, and L.Z. Holland, A retinoic acid-Hox hierarchy controls both anterior/posterior patterning and neuronal specification in the developing central nervous system of the cephalochordate amphioxus. Developmental Biology, 2006. 296(1): 190-202.

2006. Holland, N.D. and L.Z. Holland, Stage- and tissue-specific patterns of cell division in embryonic and larval tissues of amphioxus during normal development. Evol. Dev., 2006. 8: 142-149.

2006. Holland, L.Z., A SINE in the genome of the cephalochordate amphioxus is an Alu element. Int. J. Biol. Sci., 2006. 2: 61-65. 36.

2006. Langeland, J.A., L.Z. Holland, R. Chastain, and N.D. Holland, An amphioxus LIM-homeobox gene, AmphiLim1/5, expressed early in the invaginating organizer region and later in differentiating cells of the kidney and central nervous system. Int. J. Biol. Sci., 2006. 2: 110-116.

2006. Lin, H.C., L.Z. Holland, and N.D. Holland, Expression of the AmphiTcf Gene in amphioxus: insights into the evolution of the TCF/LEF gene family during vertebrate evolution 3396-3403. Dev. Dynam., 2006. 235: 3396-3403.

2006. Beaster-Jones, L., A.C. Horton, J.J. Gibson-Brown, N.D. Holland, and L.Z. Holland, The amphioxus T-box gene, AmphiTbx15/18/22, illuminates the origins of chordate segmentation. Evol. Dev., 2006. 8: 119-129.

2007. Rasmussen, S.L.K., L.Z. Holland, M. Schubert, L. Beaster-Jones, and N.D. Holland, Amphioxus AmphiDelta: evolution of Delta protein structure, segmentation, and neurogenesis. genesis, 2007. 45: 113-122.

2007. Holland, L.Z. and N.D. Holland, A revised fate map for amphioxus and the evolution of axial patterning in chordates. Int. Comp. Biol., 2007. 47: 360-372.

2007. Beaster-Jones, L., M. Schubert, and L.Z. Holland, Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer. Mech. Dev., 2007. 124: 523-542.

2007. Yu, J.-K., Y. Satou, N.D. Holland, T. Shin-I, Y. Kohara, N. Satoh, M. Bronner-Fraser, and L.Z. Holland, Axial patterning in cephalochordates and the evolution of the organizer. Nature, 2007. 445: 613-617.

2007. Holland, L.Z., A chordate with a difference. Nature, 2007. 447: 153-155.

2007. Kozmik, Z., N.D. Holland, J. Kreslova, D. Oliveri, M. Schubert, K. Jonasova, L.Z. Holland, M. Pestarino, V. Benes, and S. Candiani, Pax-Six-Eya-Dach network during amphioxus development: conservation in vitro but context specificity in vivo. Dev. Bio.. 2007. 306: 143-159.

2008. Holland, L.Z., N.D. Holland, and E. Gilland, Amphioxus and the evolution of head segmentation. Int. Comp. Biol., 2008. In Press.

2008. Holland, N.D., T.G. Campbell, J.R. Garey, L.Z. Holland, and N.G. Wilson, The Florida amphioxus (Cephalochordata) hosts larvae of the tapeworm Acanthobothrium brevissime: natural history, anatomy and taxonomic identification of the parasite. Acta Zool. (Stockh.), 2008. In press.

2008. Holland, L.Z. and S. Short, Gene duplication, co-option and recruitment during the origin of the vertebrate brain from the invertebrate chordate brain. Brain Behav. Evol., 2008. 72: 91-105.

2008. Yu, J.-K., M.-C. Wang, T. Shin-I, Y. Kohara, L. Holland, N. Satoh, and Y. Satou, A cDNA resource for the cephalochordate amphioxus Branchiostoma floridae. Development Genes and Evolution, in press.

2009. Yu, J.K., Cephalochordates (Amphioxus, or Lancelets): A Model for Understanding the Evolution of Chordate Characters, in Emerging Model Organisms. 2009, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. In press.