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HOLLAND, NICHOLAS

Professor of Marine Biology

Degrees:

• B.A., Carleton College, Minnesota
• Ph.D., Stanford University

Research Interests:

• Developmental evolution (using comparative molecular genetics to establish body part homologies between distantly related animals), with attention to the origin of the vertebrates from the invertebrates

Courses Recently Taught:

• SIO 290: Marine Biology
• SIO 30: The Oceans
• SIO290: Marine Biology
• SIO30: The Oceans

Mentoring:

• He, Xiao (Masters; 2002-2003)
• Yu, Jr-Kai (Graduate; 2000-2005)
• Schubert, Michael (Graduate; 1997-2001)
• Krug, Patrick (Graduate; 1992-1998)
• Leonard, Alexander (Graduate; 1985-1992)
• Davis, Heide (Masters; 1970-1971)
• Ermak, Thomas (Graduate; 1969-1975)
• Lusk, Gordon (Masters; 1968-1972)
• Delmonte, Peter (Masters; 1967-1969)
• Clifford, Charles (Masters; 1966-1969)
• Karig, Joane (Graduate; 1966-1973)
• Szmant, Alina (Masters; 1966-1970)

Stokes, M. D. and N. D. Holland. The lancelet. Amer. Sci. 86: 552-560,1998.

Holland, N. D. and L. Z. Holland. Amphioxus and the utility of molecular genetic data for hypothesizing body part homologies between distantly related animals. Amer. Zool. 39: 630-640, 1999.

Venkatesh, T. V., N. D. Holland, L. Z. Holland, M.-T. Su and R. Bodmer. Sequence and developmental expression of amphioxus AmphiNk2-1: insights into the evolutionary origin of the vertebrate thyroid gland and forebrain. Dev. Genes Evol. 209: 254-259, 1999.

Kozmik, Z., N. D. Holland, A. Kalousova, J. Paces, M. Schubert and L. Z. Holland. Characterization of an amphioxus paired box gene, AmphiPax2/5/8: developmental expression patterns in optic support cells, nephridium, thyroid-like structures and pharyngeal gill slits,but not in the midbrain-hindbrain boundary region. Development 126: 1295-1304, 1999.

Holland, L. Z., M. Schubert, Z. Kozmik and N. D. Holland. AmphiPax3/7, an amphioxus paired box gene: insights into chordate myogenesis, neurogenesis, and the possible evolutionary precursor of definitive vertebrate neural crest. Evol. Dev. 1: 153-165, 1999.

Holland, L. Z. and N. D. Holland. Chordate origins of the vertebrate central nervous system. Curr. Opin. Neurobiol. 9: 596-602, 1999.

Schubert, M., L. Z. Holland and N. D. Holland. Characterization of two amphioxus Wnt genes (AmphiWnt4 and AmphiWnt7b) with early expression in the developing central nervous system. Dev. Dynam. 217: 205-215, 2000.

Schubert, M., L. Z. Holland, G. D. Panopoulou, H. Lehrach and N. D. Holland. Characterization of amphioxus AmphiWnt8: insights into the evolution of patterning of the embryonic dorsoventral axis. Evol. Dev. 2: 85-92, 2000.

Schubert, M., L. Z. Holland and N. D. Holland. Characterization of an amphioxus Wnt gene, AmphiWnt11, with possible roles in myogenesis and tail outgrowth. genesis 27: 1-5, 2000.

Holland, L. Z., N. D. Holland and M. Schubert. Developmental expression of AmphiWnt1, an amphioxus gene in the Wnt1/wingless subfamily. Dev. Genes Evol. 210: 522-524, 2000.

Schubert, M., L. Z. Holland, N. D. Holland and D. K. Jacobs. A phylo-genetic tree of the Wnt genes based on all available full-length sequences, including five from the cephalochordate amphioxus. Mol. Biol. Evol. 17: 1896-1903, 2000.

L. Z. Holland, M. Schubert, N. D. Holland and T. Neuman. Evolutionary conservation of the presumptive neural plate markers amphiSox1//2/3 and AmphiNeurogenin in the invertebrate chordate amphioxus. Dev. Biol. 226: 18-33, 2000.

Langlois, M. C., J. M. Vanacker, N. D. Holland, H. Escriva, C. Queva, V. Laudet and L. Z. Holland. AmphicoupTF, a nuclear orphan receptor of the lancelet Branchiostoma floridae, is implicated in retinoic acid signalling pathways. Dev. Genes Evol. 210: 471-482, 2000.

Holland, L. Z. and N. D. Holland. Amphioxus and the evolutionary origin of the vertebrate neural crest and midbrain/hindbrain boundary. In Major Events in Early Vertebrate Evolution, P. Ahlberg, ed., Taylor and Francis, London, pp. 15-32, 2000.

Holland, N. D. and J. Y. Chen. Origin and early evolution of the vertebrates: New insights from advances in molecular biology, anatomy, and Palaeontology. Bioessays 23: 142-151. 2001.

Holland, L.Z., L.A. Rached, R. Tamme, N. D. Holland, H. Inoko, T. Shiina, C. Burgtorf, and M. Lardelli. Characterization and developmental expression o the amphioxus homolog of Notch (AmphiNotch): Evolutionary conservation of multiple expression domains in amphioxus and vertebrates. Dev. Biol. 232: 493-507, 2001.

Kozmik, Z., L. Z. Holland, M. Schubert, T. C. Lacalli, J. Kreslova, C. Vlcek, and N. D. Holland. Characterization of amphioxus AmphiVent, an evolutionarily conserved marker for chordate ventral mesoderm. genesis 29: 172-179, 2001.

Schubert, M., L. Z. Holland, M. D. Stokes, and N. D. Holland. Three amphioxus Wnt genes (AmphiWnt3, AmphiWnt5, and AmphiWnt6) associated with the tail bud: the evolution of somitogenesis in chordates. Dev. Biol. 240: 262-273, 2001.

Yu, J. K., L. Z. Holland, M. Jamrich, I. L. Blitz, and N. D. Holland. AmphiFoxE4 , an amphioxus winged helix/forkhead gene encoding a protein closely related to vertebrate thyroid transcription factor-2: expression during pharyngeal development. Evol. Dev. 4: 9-15, 2002.

Escriva, H., N. D. Holland, H. Gronemayer, V. Laudet, L. Z. Holland. The retinoic acid signalling pathway regulates anterior/posterior patterning in the nerve cord and pharynx of amphioxus, a chordate lacking neural crest. Development, 129: 2905-2916, 2002.

Yu, J. K., L.Z. Holland, N. D. Holland. An amphioxus nodal gene (AmphiNodal) with early symmetrical expression in the organizer and mesoderm and later asymmetrical expression associated with left-right axis formation. Evol. Dev. 4: 418-425. 2002.

Yu, J. K., N. D. Holland, L. Z. Holland. An amphioxus winged helix/forkhead gene, AmphiFoxD: insights into vertebrate neural crest evolution. Dev. Dynam. 225: 289-297, 2002.

Holland, N. D. and J. K. Yu. Epidermal receptor development and sensory pathways in vitally stained amphioxus (Branchiostoma floridae). Acta Zool. Stockh. 83: 309-320, 2002.

Yu, J. K., Holland, N. D., and Holland, L. Z. AmphiFoxQ2, a novel winged helix/forkhead gene, exclusively marks the anterior end of the amphioxus embryo. Dev. Genes Evol. 213: 102-105, 2003.

Holland, N. D., Venkatesh, T. V., Holland, L. Z., Jacobs, D. K., and Bodmer, R. AmphiNk2-tin, an amphioxus gene expressed in myocardial progenitors: insights into evolution of the vertebrate heart. Dev. Biol. 255: 128-137, 2003.

Schubert, M., Meulemans, D., Bronner-Fraser, M., Holland, L. Z., Holland, N. D. Differential mesodermal expression of two amphioxus MyoD family members (AmphiMRF1 and AmphiMRF2). Gene Expr. Pat. 3: 199-202, 2003.

Mallatt, J., Chen, J. Y., Holland, N. D. Comment on “a new species of yunnanozoan with implications for deuterostome evolution.” Science 300: 1372 only, 2003.

Holland, N. D. Early central nervous sytem evolution: an era of skin brains? Nature Rev. Neurosci. 4: 617-627, 2003.

Mazet, F., Masood, S., Luke, G. N., Holland, N. D., Shimeld, S. M. Expression of AmphiCoe, an amphioxus COE/EBF gene, in the developing central nervous system and epidermal sensory neurons. Genesis 38: 58-65, 2004.

Schubert, M., Holland, N. D., Escriva, H., Holland, L. Z, Laudet, V. Retinoic acid influences anteroposterior patterning of sensory neurons and their gene expression in a developing chordate (amphioxus). Proc. Natl. Acad. Sci. USA 101: 10320-10325, 2004.

Yu, J. K., Holland, N. D., Holland, L. Z. Tissue-specific expression of FoxD reporter constructs in amphioxus embryos. Dev. Biol. 274: 452-461, 2004.

Schubert, M., Yu, J. K., Holland, N. D., Escriva, H., Laudet, V., Holland, L. Z. Retinoic acid signaling acts via Hox1 to establish the posterior limit of the pharynx in the chordate amphioxus. Development 132: 61-73, 2005.

Holland, N. D., Clague, D. A., Gordon, D. P., Gebruk, A., Pawson, D. L., Vecchione, M. Lophenteropneust‚ hypothesis refuted by collection and photos of new deep-sea hemichordates. Nature 434: 374-376, 2005.

Bardet, P. L., Schubert, M.. Horad, B., Holland, L. Z., Laudet, V., Holland, N. D., Vanacker, J. M. Expression of estrogen-receptor related receptors in amphioxus and zebrafish: implications for the evolution of posterior brain segmentation at the invertebrate-to-vertebrate transition. Evol. Dev. 7: 223-233, 2005.

Smith, K. L., Holland, N. D., Rhhl, H. A. Enteropneust production of spiral fecal trails on the deep-sea floor observed with time-lapse photography. Deep-Sea Res. (Part 1) 52: 1228-1240; 2005.

Shimeld, S. M., Holland, N. D. Amphioxus molecular biology: insights into vertebrate evolution and developmental mechanisms. Can. J. Zool. 83: 90-100; 2005.

Holland, L. Z, Panfilio, K. A., Chastain, R., Schubert, M., Holland, N. D. Nuclear b-catenin promotes non-neural ectoderm and posterior cell fates in amphioxus embryos. Dev. Dynam, 233: 1430-1443, 2005.

Holland, N. D. Chordates. Curr. Biol: 15: R911-R914, 2005.

Beaster-Jones, L, Horton, A. C., Gibson-Brown, J. J., Holland, N. D., Holland, L. Z. The amphioxus T-box gene, AmphiTbx15/18/22, illuminates the origins of chordate segmentation. Evol. Dev. 8: 119-129, 2006.

Holland, N. D., Holland, L. Z. Stage- and tissue-specific patterns of cell division in embryonic and larval tissues of amphioxus during normal development. Evol. Dev. 8: 142-149, 2006.

Schubert; M., Holland; N. D., Laudet, V., Holland, L. Z. A retinoic acid-Hox hierarchy controls both anterior/posterior patterning and neuronal specification in the developing central nervous system of the cephalochordate amphioxus. Dev. Biol. 296: 190-202, 2006.

Langeland, J. A., Linda Z. Holland, L. Z., Chastain, R. A., and Holland, N. D. An amphioxus LIM-homeobox gene, AmphiLim1/5, expressed early in the invaginating organizer region and later in differentiating cells of the kidney and central nervous system. Int. J. Biol. Sci. 2: 110-116, 2006.

Bertrand, S. ,Camasses, A., Paris, M., Holland, N. D., Escriva, H. Phylogenetic analysis of amphioxus genes of the proprotein convertase family, including aPC6C, a marker of epithelial fusions during embryology. Int. J. Biol. Sci. 2: 125-132, 2006.

Castro, L. F. C., Rasmussen, S. L. K., Holland, P. W. H., Holland, N. D., Holland, L. Z. A Gbx homeobox gene in amphioxus: insights into ancestry of the ANTP class and evolution of the midbrain/hindbrain boundary. Dev. Biol. 295: 40-51, 2006.

Lin, H. C. Holland, L. Z., Holland, N. D. Expression of the AmphiTcf Gene in amphioxus: insights into the evolution of the TCF/LEF gene family during vertebrate evolution. Dev. Dynam. 235: 3396-3403, 2006.

Yu, J. K., Satou, Y., Holland, N. D., Shin-I. T., Kohara, Y., Satoh, N., Bronner-Fraser, M., Holland, L. Z. Axial patterning in cephalochordates and the evolution of the organizer. Nature 445: 613-617, 2007.

Rasmussen, S. L. K., Holland, L. Z., Schubert, M., Beaster-Jones, L., Holland, N. D. Amphioxus AmphiDelta Evolution of Delta protein structure, segmentation, and neurogenesis. Genesis 45: 113-122, 2007.

Kozmik, Z., Holland, N. D., Kreslova, J., Oliveri, D., Schubert, M., Jonasova, K., Holland, L. Z., Pestarino, M., Benes, V., Candiani, S. Pax-Six-Eya-Dach network during amphioxus development: conservation in vitro but context-specificity in vivo. Dev. Biol. 306: 143-159, 2007.

Fuentes, M., Benito, E., Bertrand, S., Paris, M, Mignardot, A., Godoy, G., Jimenez-Delgado, S., Oliveri,, D., Candiani, S., Hirsinger, E., D‚Aniello, S., Pascual-Anaya, J., Pestarino, M., Vernier, P.,Nicolas, J. F., Schubert, M., Laudet, V., Geneviere, A. M., Ricard Albalat, R., Garcia-Fernandez, J., Holland, N. D., Escriva, H. Insights into spawning behaviour and development of the Mediterranean amphioxus (Branchiostoma lanceolatum). J. Exp. Zool. B. 308: 484-493, 2007.

Deheyn, D. D.,Holland, N. D., Kubokawa, K., McCarthy, J. K., Murakami, A., Porrachia, M., Rouse, G. W. Endogenous green fluorescent protein (FP) in amphioxus. Biol. Bull. 213: 95-100, 2007.

Holland, L. Z., Holland, N. D. A revised fate map for amphioxus and the evolution of axial patterning in chordates. Integrat.Comp. Biol. 47: 360-372, 2007

Holland, N. D. Hagfish embryos again–the end of a long drought. BioEssays 29: 833-836, 2007.

Candiani, S., Holland, N. D., Oliveri, D., Parodi, M., Pestarino, M. Expression of the amphioxus Pit-1 gene (AmphiPou1F1/Pit-1) exclusively in the developing preoral organ, a putative homolog of the vertebrate adenohypophysis. Brain Res. Bull. 75: 324-330, 2008

Paris, M., Escriva, H., Schubert, M., Brunet, F., Brtko, J., Ciesielski, F., Roecklin, D., Vivat-Hannah, V., Jamin, E. L., Cravedi, J. P., Scanlan, T. S., Renaud, J. P., Holland, N. D., Laudet, V. Amphioxus postembryonic development reveals the homology of chordate metamorphosis. Curr. Biol. 18: 825-830, 2008